Some subdivisions extend through more than one region and division. Only the portion of the subdivision in this division is shown.
| Subdivision | From | To | Length | Spurs | Authority | Time Table |
|---|---|---|---|---|---|---|
| Fergus | Lynden, Ontario | Palmerston, Ontario | 72.8 miles | 10 | TT&TO | Fergus |
| Dundas | Paris West, Ontario | London, Ontario | 44.7 miles | 15 | ABS | Dundas |
| Talbot | Walton junction, Ontario | Port Stanley, Ontario | 23.6 miles | 2 | TT&TO | Talbot |
| Cayuga | Feeder West, Ontario | ST.Thomas, Ontario | 98.1 miles | 10 | TT&TO | Cayuga |
| Drumbo | Bright, Ontario | Stratford, Ontario | 17.4 miles | 0 | TT&TO | Drumbo |
| Thorndale | Stratford, Ontario | London Junction, Ontario | 31.3 miles | 4 | TT&TO | Thorndale |
| Strathroy | London, Ontario | Sarnia, Ontario | 58.9 miles | 1 | ABS & CTC | Strathroy |
| Newton | Stratford, Ontario | Palmerston, Ontario | 36.6 miles | 1 | TT&TO | Newton |
| Owen Sound | Palmerston, Ontario | Owen Sound, Ontario | 71.4 miles | 4 | TT&TO | Owen Sound |
| Southampton | Harris Junction, Ontario | Douglas Point, Ontario | 59.6 miles | 2 | TT&TO | Southampton |
| Kincardine | Listowel, Ontario | Kinkardine, Ontario | 57.5 miles | 1 | TT&TO | Kincardine |
| Goderich | Stratford, Ontario | Goderich, Ontario | 45.9 miles | 2 | TT&TO | Goderich |
| Forest | ST.Mary’s Junction, Ontario | Sarnia Junction, Ontario | 70.6 miles | 0 | TT&TO | Forest |
| Chatham | St Thomas, Ontario | Windsor Yard, Ontario | 107.2 miles | 7 | TT&TO 2 tracks | Chatham |
| Longwood | Komoka, Ontario | Glencoe, Ontario | 20.7 miles | 0 | TT&TO | Longwood |
| ST. Clair Tunnel | Sarnia, Ontario | Port Huron, Mi, USA | 3.1 miles | 2 | CTC | St. Clair Tunnel |
| Exeter | Hyde Park, Ontario | Clinton Junction, Ontario | 45.5 miles | 0 | TT&TO | Exeter |
| Guelph | Acton, Ontario | Stratford, Ontario | 53 miles | 5 | TT&TO | Guelph |